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Microbial Pathogenesis
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Research
Research at a Glance
Publications Timeline
A big-picture view of Dmitrii Mazurov's research output by year.
49Publications
778Citations
Publications
2024
Efficient Genome Editing Using ‘NanoMEDIC’ AsCas12a-VLPs Produced with Pol II-Transcribed crRNA
Borovikova S, Shepelev M, Mazurov D, Kruglova N. Efficient Genome Editing Using ‘NanoMEDIC’ AsCas12a-VLPs Produced with Pol II-Transcribed crRNA. International Journal Of Molecular Sciences 2024, 25: 12768. PMID: 39684477, PMCID: PMC11641575, DOI: 10.3390/ijms252312768.Peer-Reviewed Original ResearchConceptsRNA ribonucleoprotein complexVirus-like particlesGenome editingProcesses pre-crRNARNA polymerase IIIEfficient genome editingTargeted genome editingJurkat T cellsGene therapy applicationsMature crRNAsPre-crRNAPolymerase IIICas nucleasesRibonucleoprotein complexEfficient targeted genome editingCrRNAMammalian cellsLevels of gene editingPackaging mechanismPromoter usageU6 promoterT cellsEditing efficiencyCXCR4 knockoutGene editingLymphocyte phosphatase-associated phosphoprotein (LPAP) as CD45 protein stability regulator
Kruglova N, Mazurov D, Filatov A. Lymphocyte phosphatase-associated phosphoprotein (LPAP) as CD45 protein stability regulator. Биохимия 2024, 89: 897-907. DOI: 10.31857/s0320972524050118.Peer-Reviewed Original ResearchConceptsA New Chimeric Antibody against the HIV-1 Fusion Inhibitory Peptide MT-C34 with a High Affinity and Fc-Mediated Cellular Cytotoxicity
Kalinichenko S, Ramadan L, Kruglova N, Balagurov K, Lukashina M, Mazurov D, Shepelev M. A New Chimeric Antibody against the HIV-1 Fusion Inhibitory Peptide MT-C34 with a High Affinity and Fc-Mediated Cellular Cytotoxicity. Biology 2024, 13: 675. PMID: 39336102, PMCID: PMC11428423, DOI: 10.3390/biology13090675.Peer-Reviewed Original ResearchConceptsCellular cytotoxicityHIV-1Chimeric antibodyInhibitors of HIV-1 entryAntibody-dependent cellular cytotoxicityHIV-1 infectionHIV-1 entryRecombinant chimeric antibodyHumanized antibody trastuzumabMouse monoclonal antibodyCXCR4 locusCD4 lymphocytesCD4 cellsParental mouse monoclonal antibodyCAR cellsGeneration of antibodiesAntibody trastuzumabMonoclonal antibodiesAntibodiesMouse hybridomasConstant regionKnock-inCellsCytotoxicityPeptideDonor DNA Modification with Cas9 Targeting Sites Improves the Efficiency of MTC34 Knock-in into the CXCR4 Locus
Shepelev M, Komkov D, Golubev D, Borovikova S, Mazurov D, Kruglova N. Donor DNA Modification with Cas9 Targeting Sites Improves the Efficiency of MTC34 Knock-in into the CXCR4 Locus. Molecular Biology 2024, 58: 672-682. DOI: 10.1134/s0026893324700250.Peer-Reviewed Original ResearchConceptsCas9 target sitesDouble-strand breaksKnock-inCell genomeGenetic constructsDNA modificationsDonor DNADonor plasmid DNATarget siteKnock-in efficiencyGenome editing technologyInduce double-strand breaksProximal nucleotidesPAM sitesDonor plasmidDonor sequenceCXCR4 locusGenomeIn vitroInduced cleavageCRISPR/Cas9 systemCas9LociEditing technologyDNAMethods to Increase the Efficiency of Knock-in of a Construct Encoding the HIV-1 Fusion Inhibitor, MT-C34 Peptide, into the CXCR4 Locus in the CEM/R5 T Cell Line
Golubev D, Komkov D, Shepelev M, Mazurov D, Kruglova N. Methods to Increase the Efficiency of Knock-in of a Construct Encoding the HIV-1 Fusion Inhibitor, MT-C34 Peptide, into the CXCR4 Locus in the CEM/R5 T Cell Line. Molecular Biology 2024, 58: 658-671. DOI: 10.1134/s0026893324700249.Peer-Reviewed Original ResearchConceptsNuclear localization signalNonhomologous end joiningDNA nuclear targeting sequencesKnock-inCXCR4 locusDNA repairT cell linesNonhomologous end-joining pathwayNuclear targeting sequenceDNA-dependent protein kinase inhibitorBlock DNA repairHIV-1Knock-in efficiencyEffective gene therapy approachGenome editing technologyTranscription factor NF-kBLocalization signalTreat HIV infectionGene therapy approachesTarget sequenceDonor plasmidCas9 nucleaseCas9 proteinEnd joiningDNA modificationsEfficient editing of the CXCR4 locus using Cas9 ribonucleoprotein complexes stabilized with polyglutamic acid
Golubev D, Komkov D, Shepelev M, Mazurov D, Kruglova N. Efficient editing of the CXCR4 locus using Cas9 ribonucleoprotein complexes stabilized with polyglutamic acid. Доклады Российской Академии Наук Науки О Жизни 2024, 514: 85-90. DOI: 10.31857/s2686738924010164.Peer-Reviewed Original ResearchLymphocyte Phosphatase-Associated Phosphoprotein (LPAP) as a CD45 Protein Stability Regulator
Kruglova N, Mazurov D, Filatov A. Lymphocyte Phosphatase-Associated Phosphoprotein (LPAP) as a CD45 Protein Stability Regulator. Biochemistry (Moscow) 2024, 89: 912-922. PMID: 38880651, DOI: 10.1134/s0006297924050110.Peer-Reviewed Original ResearchMeSH Keywords and ConceptsConceptsLymphocyte phosphatase-associated phosphoproteinExpression level of CD45Protein stability regulationSurface expression of CD45Expression of CD45Levels of CD45Binding partnersPhosphatase CD45Lymphoid cellsLymphocyte activationCD45 proteinSurface expressionCD45Biochemical evidenceK562 cellsStability regulationLymphocytesPhosphoproteinThe RRE-REV module has no effect on the packaging efficiency of cas9 and Gag proteins into nanomedic virus-like particles
Kruglova N, Komkov D, Mazurov D, Shepelev M. The RRE-REV module has no effect on the packaging efficiency of cas9 and Gag proteins into nanomedic virus-like particles. Доклады Российской Академии Наук Науки О Жизни 2024, 515: 64-70. DOI: 10.31857/s2686738924020121.Peer-Reviewed Original ResearchConceptsRev expression plasmidVirus-like particlesEmpty control plasmidGene therapy of human diseasesGag proteinTherapy of human diseasesGene therapyViral Gag proteinTarget cellsControl plasmidProtein levelsCas9 nucleaseGenome editingGagEfficiency of genome editingMethods of genome editingExpression of Cas9Plasmid constructsCotransfectionHuman diseasesPlasmidCell lysatesNuclear exportAlternative substitutions of N332 in HIV-1AD8 gp120 differentially affect envelope glycoprotein function and viral sensitivity to broadly neutralizing antibodies targeting the V3-glycan
Jeffy J, Parthasarathy D, Ahmed S, Cervera-Benet H, Xiong U, Harris M, Mazurov D, Pickthorn S, Herschhorn A. Alternative substitutions of N332 in HIV-1AD8 gp120 differentially affect envelope glycoprotein function and viral sensitivity to broadly neutralizing antibodies targeting the V3-glycan. MBio 2024, 15: e02686-23. PMID: 38470051, PMCID: PMC11005340, DOI: 10.1128/mbio.02686-23.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsHIV-1 strainsHIV-1V3-glycanEnv antigensHIV-1 infection <i>inNeutralizing antibodiesHost CD4<sup>+</sup> T cellsCirculating HIV-1 strainsEnvelope glycoproteinCD4<sup>+</sup> T cellsHuman immunodeficiency virus type IHIV-1 envelope glycoproteinBlock HIV-1 infectionHIV-1 Env trimerHIV-1 isolatesHIV-1 infectionGp120 V3 loopTarget of neutralizing antibodiesHIV-1 entryCell surface expressionLevels of cell surface expressionSensitivity to antibodiesN332 glycanSoluble CD4Gp120 shedding
2023
Ultrasensitive quantification of HIV-1 cell-to-cell transmission in primary human CD4+ T cells measures viral sensitivity to broadly neutralizing antibodies
Mazurov D, Herschhorn A. Ultrasensitive quantification of HIV-1 cell-to-cell transmission in primary human CD4+ T cells measures viral sensitivity to broadly neutralizing antibodies. MBio 2023, 15: e02428-23. PMID: 38063394, PMCID: PMC10790777, DOI: 10.1128/mbio.02428-23.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsHIV-1 cell-to-cell transmissionCell-to-cell transmissionHIV-1Neutralizing antibodiesHIV-1 escapeHIV-1 transmissionHIV-1 resistanceHuman T cellsT cellsClinical trialsMode of transmissionViral sensitivityUltrasensitive assayEvaluation of pre-existingAntibodiesAssayCellsPre-existingRetrovirusesTrials