Characterization and Subcellular Localization of Human Pmel 17/silver, a 100-kDa (Pre)Melanosomal Membrane Protein Associated With 5,6,-Dihydroxyindole-2-Carboxylic Acid (DHICA) Converting Activity
Lee Z, Hou L, Moellmann G, Kuklinska E, Antol K, Fraser M, Halaban R, Kwon B. Characterization and Subcellular Localization of Human Pmel 17/silver, a 100-kDa (Pre)Melanosomal Membrane Protein Associated With 5,6,-Dihydroxyindole-2-Carboxylic Acid (DHICA) Converting Activity. Journal Of Investigative Dermatology 1996, 106: 605-610. PMID: 8617992, DOI: 10.1111/1523-1747.ep12345163.Peer-Reviewed Original ResearchConceptsPmel 17Baculovirus expression vectorCo-precipitated proteinsElectron transfer chainPigmentation lociCytosolic vesiclesInsect cellsComplexed proteinsSubcellular localizationPolyclonal antibodiesProtein AssociatedMelanin biosynthesisPigment cellsExpression vectorPrimary structureTransfer chainApproximate molecular sizeHuman melanoma cellsProteinNatural proteinsOxidoreductive enzymesMelanoma cellsUltrastructural locationElectron microscopic cytochemistryCells
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