Yevheniia Ishchenko
she/her/hers
Associate Research ScientistCards
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Research
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Featured Publications
Microglial amyloid beta clearance is driven by PIEZO1 channels
Ishchenko Y, Jäntti H, Sitnikova V, Shakirzyanova A, Giudice L, Ugidos I, Gómez-Budia M, Korvenlaita N, Ohtonen S, Belaya I, Fazaludeen F, Mikhailov N, Gotkiewicz M, Ketola K, Lehtonen Š, Koistinaho J, Kanninen K, Hernández D, Pébay A, Giugno R, Korhonen P, Giniatullin R, Malm T. Microglial amyloid beta clearance is driven by PIEZO1 channels. Journal Of Neuroinflammation 2022, 19: 147. PMID: 35706029, PMCID: PMC9199162, DOI: 10.1186/s12974-022-02486-y.Peer-Reviewed Original ResearchThe viral protein corona directs viral pathogenesis and amyloid aggregation
Ezzat K, Pernemalm M, Pålsson S, Roberts T, Järver P, Dondalska A, Bestas B, Sobkowiak M, Levänen B, Sköld M, Thompson E, Saher O, Kari O, Lajunen T, Sverremark Ekström E, Nilsson C, Ishchenko Y, Malm T, Wood M, Power U, Masich S, Lindén A, Sandberg J, Lehtiö J, Spetz A, EL Andaloussi S. The viral protein corona directs viral pathogenesis and amyloid aggregation. Nature Communications 2019, 10: 2331. PMID: 31133680, PMCID: PMC6536551, DOI: 10.1038/s41467-019-10192-2.Peer-Reviewed Original ResearchMeSH KeywordsAlzheimer DiseaseAmyloid beta-PeptidesAnimalsBronchoalveolar Lavage FluidCell Line, TumorChlorocebus aethiopsDisease Models, AnimalFemaleHealthy VolunteersHerpes SimplexHerpesvirus 1, HumanHost-Pathogen InteractionsHumansMaleMiceMice, TransgenicPeptide FragmentsProtein AggregatesProtein CoronaRespiratory Syncytial Virus InfectionsRespiratory Syncytial Virus, HumanVero CellsConceptsProtein coronaRespiratory syncytial virusArtificial nanoparticlesProtein corona layerBiological fluidsDifferent biological fluidsImmune cell activationCorona layerSyncytial virusAmyloid plaquesObligate intracellular parasitesSimplex virusAmyloid β-peptideAnimal modelsViral-host interactionsHSV-1Cell activationViral pathogenesisAlzheimer's diseaseNanoparticlesType 1Viral infectivityIntracellular parasitesVirusDiseaseReconstructed Serine 288 in the Left Flipper Region of the Rat P2X7 Receptor Stabilizes Nonsensitized States
Ishchenko Y, Novosolova N, Khafizov K, Bart G, Timonina A, Fayuk D, Skorinkin A, Giniatullin R. Reconstructed Serine 288 in the Left Flipper Region of the Rat P2X7 Receptor Stabilizes Nonsensitized States. Biochemistry 2017, 56: 3394-3402. PMID: 28616989, DOI: 10.1021/acs.biochem.7b00258.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SubstitutionAnimalsBinding SitesBiological Transport, ActiveComputer SimulationFluorescent DyesHEK293 CellsHumansKineticsLigandsModels, MolecularPatch-Clamp TechniquesPoint MutationProtein Interaction Domains and MotifsProtein StabilityPurinergic P2X Receptor AgonistsPurinergic P2X Receptor AntagonistsRatsReceptors, Purinergic P2X7Recombinant ProteinsSerineConceptsRat P2X7 receptorP2X7 receptorSerine 288Novel phenotypesCorresponding residuesPosition 288MM ATPHuman P2X7 receptorMolecular modeling dataF288Agonist bindingATPReceptor statesP2X receptorsReceptor desensitizationPhenotypeATP concentrationFast deactivation ratesResiduesReceptorsKey roleS applicationMutantsSerineWT valuesSelective Calcium-Dependent Inhibition of ATP-gated P2X3 Receptors by Bisphosphonates-induced Endogenous ATP analogue ApppI
Ishchenko Y, Shakirzyanova A, Giniatullina R, Skorinkin A, Bart G, Turhanen P, Määttä J, Mönkkönen J, Giniatullin R. Selective Calcium-Dependent Inhibition of ATP-gated P2X3 Receptors by Bisphosphonates-induced Endogenous ATP analogue ApppI. Journal Of Pharmacology And Experimental Therapeutics 2017, 361: jpet.116.238840. PMID: 28404687, DOI: 10.1124/jpet.116.238840.Peer-Reviewed Original ResearchConceptsNitrogen-containing bisphosphonatesP2X3 receptorsP2X7 receptorBone cancerInhibitory actionBone resorptive disordersNodose ganglion neuronsRat sensory neuronsPrimary bone cancerRat P2X3 receptorsPatch-clamp techniqueWeak agonist activityCalcium-dependent inhibitionBone metastasesTrigeminal neuronsPurinergic mechanismsUnbearable symptomsGanglion neuronsReceptor subtypesSensory neuronsP2X receptorsPathologic processesAgonist activityHuman P2X3Human embryonic kidney cellsStable, synthetic analogs of diadenosine tetraphosphate inhibit rat and human P2X3 receptors and inflammatory pain
Viatchenko-Karpinski V, Novosolova N, Ishchenko Y, Azhar M, Wright M, Tsintsadze V, Kamal A, Burnashev N, Miller A, Voitenko N, Giniatullin R, Lozovaya N. Stable, synthetic analogs of diadenosine tetraphosphate inhibit rat and human P2X3 receptors and inflammatory pain. Molecular Pain 2016, 12: 1744806916637704. PMID: 27030723, PMCID: PMC4955970, DOI: 10.1177/1744806916637704.Peer-Reviewed Original ResearchConceptsInflammatory painAntinociceptive effectChronic painHigh-affinity desensitizationPeripheral sensory neuronsRat brain cellsWeak partial agonistHuman P2X3 receptorsPotent analgesic agentVivo inflammation modelsDiadenosine tetraphosphateWhole-cell currentsWeak partial agonismThermal hyperalgesiaP2X3 receptorsDorsal rootsPain responseSimilar inhibitory activityAnalgesic agentsBody of evidenceInflammation modelSensory neuronsBrain cellsP2X3RsPain
2022
Phenotypes of Motor Deficit and Pain after Experimental Spinal Cord Injury
Krotov V, Medvediev V, Abdallah I, Bozhenko A, Tatarchuk M, Ishchenko Y, Pichkur L, Savosko S, Tsymbaliuk V, Kopach O, Voitenko N. Phenotypes of Motor Deficit and Pain after Experimental Spinal Cord Injury. Bioengineering 2022, 9: 262. PMID: 35735505, PMCID: PMC9220047, DOI: 10.3390/bioengineering9060262.Peer-Reviewed Original ResearchSpinal cord injuryMotor deficitsMotor dysfunctionSCI ratsCord injuryNociceptive sensitivityExperimental spinal cord injuryModerate spinal cord injuryLong-term rehabilitationPain syndromeSevere complicationsMotor functionIdentical injuryPainInjuryMotor disabilityDysfunctionNeurotraumaOld animalsMechanical modalitiesTissue capabilityCommon outcomeRegression analysisDeficitsRats
2021
Regulation of the NMDA receptor by its cytoplasmic domains: (How) is the tail wagging the dog?
Ishchenko Y, Carrizales MG, Koleske AJ. Regulation of the NMDA receptor by its cytoplasmic domains: (How) is the tail wagging the dog? Neuropharmacology 2021, 195: 108634. PMID: 34097949, PMCID: PMC8410658, DOI: 10.1016/j.neuropharm.2021.108634.Peer-Reviewed Original ResearchConceptsCarboxyl-terminal domainN-methyl-D-aspartate receptorsUnique modular architectureIntracellular C-tailAmino acid sequenceDocking motifAttention deficit hyperactivity disorderKnown proteinsCytoplasmic domainC-tailTerminal domainSequence homologyAcid sequenceSynapse developmentSynaptic targetingCovalent modificationGenetic variantsGlutamate receptor subunitsAllosteric modulationImportant functionsReceptor subunitsIntellectual disabilityMetabotropic signalingSubunitsProtein
2019
PSEN1ΔE9, APPswe, and APOE4 Confer Disparate Phenotypes in Human iPSC-Derived Microglia
Konttinen H, Cabral-da-Silva M, Ohtonen S, Wojciechowski S, Shakirzyanova A, Caligola S, Giugno R, Ishchenko Y, Hernández D, Fazaludeen M, Eamen S, Budia M, Fagerlund I, Scoyni F, Korhonen P, Huber N, Haapasalo A, Hewitt A, Vickers J, Smith G, Oksanen M, Graff C, Kanninen K, Lehtonen S, Propson N, Schwartz M, Pébay A, Koistinaho J, Ooi L, Malm T. PSEN1ΔE9, APPswe, and APOE4 Confer Disparate Phenotypes in Human iPSC-Derived Microglia. Stem Cell Reports 2019, 13: 669-683. PMID: 31522977, PMCID: PMC6829767, DOI: 10.1016/j.stemcr.2019.08.004.Peer-Reviewed Original ResearchConceptsInduced pluripotent stem cellsPluripotent stem cellsErythromyeloid progenitorsDisparate phenotypesIsogenic controlsSignature genesMicroglia-like cellsHuman microglia-like cellsGenetic backgroundHuman iPSCStem cellsMicroglial signature genesYolk sacIPSC linesIntracellular CaImpairs phagocytosisIMGLsMetabolic activitySmall moleculesHigh-yield protocolCellsAPPswe mutationMinor alterationsCytokine secretionGenes
2017
Augmentation of Anticancer Drug Efficacy in Murine Hepatocellular Carcinoma Cells by a Peripherally Acting Competitive N‑Methyl‑d‑aspartate (NMDA) Receptor Antagonist
Gynther M, Silvestri I, Hansen J, Hansen K, Malm T, Ishchenko Y, Larsen Y, Han L, Kayser S, Auriola S, Petsalo A, Nielsen B, Pickering D, Bunch L. Augmentation of Anticancer Drug Efficacy in Murine Hepatocellular Carcinoma Cells by a Peripherally Acting Competitive N‑Methyl‑d‑aspartate (NMDA) Receptor Antagonist. Journal Of Medicinal Chemistry 2017, 60: 9885-9904. PMID: 29205034, PMCID: PMC5788303, DOI: 10.1021/acs.jmedchem.7b01624.Peer-Reviewed Original ResearchConceptsMurine hepatocellular carcinoma cellsHepatocellular carcinoma cellsN-methyl-D-aspartate receptor antagonistCompetitive N-methyl-D-aspartate (NMDA) receptor antagonistMultidrug resistanceCarcinoma cellsCancer cellsCommon solid tumorsNMDA receptor inhibitionIntrinsic multidrug resistanceReceptor antagonistReceptor inhibitionAnticancer drug efficacyUnderlying biological mechanismsSolid tumorsDrug efficacyReduced expressionCytotoxic actionSorafenibStructure-activity studiesMDR transportersAnticancer drugsBiological mechanismsCellsPromising strategyAge-Dependent, Subunit Specific Action of Hydrogen Sulfide on GluN1/2A and GluN1/2B NMDA Receptors
Yakovlev A, Kurmasheva E, Ishchenko Y, Giniatullin R, Sitdikova G. Age-Dependent, Subunit Specific Action of Hydrogen Sulfide on GluN1/2A and GluN1/2B NMDA Receptors. Frontiers In Cellular Neuroscience 2017, 11: 375. PMID: 29225568, PMCID: PMC5705612, DOI: 10.3389/fncel.2017.00375.Peer-Reviewed Original ResearchNMDA receptorsAge-dependent effectsT cellsNervous systemN-methyl-D-aspartate (NMDA) glutamate receptorsGlutamate receptor subunit compositionAdenylate cyclase inhibitor MDL-12330ANMDA receptor compositionAcceleration of desensitizationReceptor subunit compositionHippocampal NMDA receptorsCentral nervous systemThird postnatal weekHEK293T cellsAge-dependent switchEffect of NaHSPatch-clamp techniqueNeuroprotective rolePyramidal neuronsNMDA currentsExcessive activationGlutamate receptorsDonor sodium hydrosulfidePostnatal weekS donor sodium hydrosulfide