2019
An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms
Kanca O, Zirin J, Garcia-Marques J, Knight S, Yang-Zhou D, Amador G, Chung H, Zuo Z, Ma L, He Y, Lin W, Fang Y, Ge M, Yamamoto S, Schulze K, Hu Y, Spradling A, Mohr S, Perrimon N, Bellen H. An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms. ELife 2019, 8: e51539. PMID: 31674908, PMCID: PMC6855806, DOI: 10.7554/elife.51539.Peer-Reviewed Original Research
2018
An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila
Li-Kroeger D, Kanca O, Lee P, Cowan S, Lee M, Jaiswal M, Salazar J, He Y, Zuo Z, Bellen H. An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila. ELife 2018, 7: e38709. PMID: 30091705, PMCID: PMC6095692, DOI: 10.7554/elife.38709.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsClustered Regularly Interspaced Short Palindromic RepeatsDrosophilaEntomologyGene TargetingGenes, ReporterIntegrasesMutagenesis, InsertionalConceptsNew genetic toolsDouble headerTagged alleleGene taggingPrecise gene manipulationEndogenous genesGenetic toolsProtein functionProtein localizationMultiple downstream applicationsArtificial exonCassette exchangeProtein trappingEmbryo injectionNull allelesExpression patternsGene manipulationGene expressionDNA fragmentsDominant markersGenesCRISPRIntegration efficiencyDownstream applicationsAllelesA gene-specific T2A-GAL4 library for Drosophila
Lee P, Zirin J, Kanca O, Lin W, Schulze K, Li-Kroeger D, Tao R, Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, Housden B, Mohr S, Yamamoto S, Levis R, Spradling A, Perrimon N, Bellen H. A gene-specific T2A-GAL4 library for Drosophila. ELife 2018, 7: e35574. PMID: 29565247, PMCID: PMC5898912, DOI: 10.7554/elife.35574.Peer-Reviewed Original ResearchConceptsFunction phenotypesIntrons of genesExpression of hundredsCell-type specificityLethal insertionsEssential genesGene functionEndogenous promoterLethal mutationsCDNA constructsGene expressionInserted cassettesGenesPowerful resourceExpressionPhenotypeSignal 3DrosophilaGAL4Severe lossIntronsChromosomesTranscriptionCRISPRPromoter
2017
A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells
Nagarkar-Jaiswal S, Manivannan S, Zuo Z, Bellen H. A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells. ELife 2017, 6: e26420. PMID: 28561736, PMCID: PMC5493436, DOI: 10.7554/elife.26420.Peer-Reviewed Original Research
2015
A genetic toolkit for tagging intronic MiMIC containing genes
Nagarkar-Jaiswal S, DeLuca S, Lee P, Lin W, Pan H, Zuo Z, Lv J, Spradling A, Bellen H. A genetic toolkit for tagging intronic MiMIC containing genes. ELife 2015, 4: e08469. PMID: 26102525, PMCID: PMC4499919, DOI: 10.7554/elife.08469.Peer-Reviewed Original ResearchConceptsProtein interaction partnersGenes/proteinsProtein expression patternsUncharacterized genesGenetic toolkitIntegration cassetteMiMIC insertionsPhiC31 recombinaseProtein tagsFRT sequencesNew exonsGenetic strategiesInteraction partnersExpression patternsGenesExonsPlasmid DNAProteinMass specTagsIntronsLarge collectionGFPImmunoprecipitationMimics
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