2018
An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila
Li-Kroeger D, Kanca O, Lee P, Cowan S, Lee M, Jaiswal M, Salazar J, He Y, Zuo Z, Bellen H. An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila. ELife 2018, 7: e38709. PMID: 30091705, PMCID: PMC6095692, DOI: 10.7554/elife.38709.Peer-Reviewed Original ResearchConceptsNew genetic toolsDouble headerTagged alleleGene taggingPrecise gene manipulationEndogenous genesGenetic toolsProtein functionProtein localizationMultiple downstream applicationsArtificial exonCassette exchangeProtein trappingEmbryo injectionNull allelesExpression patternsGene manipulationGene expressionDNA fragmentsDominant markersGenesCRISPRIntegration efficiencyDownstream applicationsAlleles
2015
A genetic toolkit for tagging intronic MiMIC containing genes
Nagarkar-Jaiswal S, DeLuca S, Lee P, Lin W, Pan H, Zuo Z, Lv J, Spradling A, Bellen H. A genetic toolkit for tagging intronic MiMIC containing genes. ELife 2015, 4: e08469. PMID: 26102525, PMCID: PMC4499919, DOI: 10.7554/elife.08469.Peer-Reviewed Original ResearchConceptsProtein interaction partnersGenes/proteinsProtein expression patternsUncharacterized genesGenetic toolkitIntegration cassetteMiMIC insertionsPhiC31 recombinaseProtein tagsFRT sequencesNew exonsGenetic strategiesInteraction partnersExpression patternsGenesExonsPlasmid DNAProteinMass specTagsIntronsLarge collectionGFPImmunoprecipitationMimics
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